Research interesting:

1. Gene delivery system using viral or nonviral vectors
2. Targeting DDS using nanotechnology
3. Stem Cells used as TDDS
4. Transdermal Therapeutic system
5. Molecular Biopharmaceutics

The researches were supported by the National Natural Science Foundation of China , Joint grant supported by both JSPS Japan and NSFC China, and by the Foundation of Ministry of Education, Ministry of Health, Ministry of Personality, and so on. Published more than 150 papers and reviewsin peer review journals.Authorized 13 national patents. Honored more than 7 awards including the “Chinese Embassy Award for the Best Research Paper in Japan 2003”, “JSPS Invitation Fellowship for Research in Japan 2007”and “Distinguished Young Scholar Foundation of Zhejiang, China”. The editor board of “Journal of Cancer Therapy”, “Asian Journal of Pharmaceutical Sciences” and “Chinese Journal of Modern Applied Pharmacy ”. The invited reviewer ofJ Control Rel, Mol Pharm, Pharm Res., Biomaterials, Int J Pharm, Cytokine and so on.

Education:

1989.2-1992.12  Faculty of Pharmaceutical Sciences, Zhejiang Medical University, China

1993.9-1996.6   Department of Pharmaceutics, Zhejiang Medical University, China

          Awarded the degree of M.S.(Prof. Wen-Quan Liang)

Theme: ‘Transdermal Iontophoresis of Non-Steroidal Anti-Inflammatory Drugs’

2000.10-2001.8  North-Eastern Normal University, China

          Japanese training

• Department of Biopharmaceutics, Osaka University, Japan

Research student

•   Department of Biopharmaceutics, Osaka University, Japan

Doctoral Course Student, Ph.D (Prof. Tadanori Mayumi, Prof. Shinsaku Nakagawa)

Theme: ‘Optimization of cancer immunotherapy using a stimulating pair: chemokine and cytokine: --Synergistic anti-tumor activity induced by the combination of CCL27 and interleukin 12via recombinant gene vector’

Research and professional experiences:

:

1987.8-1993.8ResearchAssistant

             Zhejiang Medical University, China

1996.7-1998.8Assistant Professor in Pharmaceutics,

             Zhejiang Medical University, China

Lecturer in Pharmaceutics,Zhejiang University, China

Associate .Professor in Pharmaceutics, Director of the Department of Biotechnical Pharmaceutics, Vice director of the Institute of Pharmaceutics, Zhejiang University

State Food and Drug Administration in China as an expert for new       drug evaluation

2007.11-2008.2 Research Scientist, JSPS Invitation Fellowship for Research in Japan, Kyoto University, Japan  

2007.6-      SFDA China, as an invited expert for novel drug evaluation

2010.11   Prof. in Pharmaceutics, Director of the Department of Biotechnical Pharmaceutics, Vice director of the Institute of Pharmaceutics, Zhejiang University

2010.12-          Prof. in Pharmaceutics, Dean of the Department of Pharmacy, Zhejiang University, Director of the Department of Biotechnical Pharmaceutics

Publications:

More than 150 research articles or reviews

7 contributions for book chapter and 15 patents

Representative publications

Min Han, Qing Lv, Xin-Jiang Tang, Yu-Lan Hu, Dong-Hang Xu, Fan-Zhu Li, Wen-Quan Liang, Jian-Qing Gao*, Overcoming drug resistance of MCF-7/ADR cells by altering intracellular distribution of doxorubicin via MVP knockdown with a novel siRNA polyamidoamine-hyaluronic acid complex, J Control Release, 2012, 163:136-144

Xing-Lei Yao,Yasuo Yoshioka, Yu-Zhe Chen, Gui-Xing Ruan, Hiroyuki Mizuguchi, Yohe Mukai, Naoki Okada, Jian-Qing Gao^*, Shinsaku Nakagawa^*,Construction of PEGylated adenovirus vector with targeting peptides for cancer gene therapy, Biomacromolecules, 2012 Aug 13;13(8):2402-9

Yu-Lan Hu, Bing Huang, Tian-Yuan Zhang, Pei-Hong Miao, Yasuhiko Tabata,Jian-Qing Gao*, Mesenchymal stem cells as a novel carrier for targeted-delivery of gene in cancer therapy based on non-viral transfection, Mol Pharm,2012,9:2698-2709

Bing Huang, Yasuhiko Tabata, Jian-Qing Gao*, Mesenchymal stem cells as therapeutic agents and potential targeted therapy of brain diseases,J Control Release, 2012,162:464-473

Min Han, Yuan-Yuan Diao, Hong-Liang Jiang, Xiao-Ying Ying, Da-Wei Chen, Wen-Quan Liang, Jian-Qing Gao*, Molecular mechanism study of chemosensitization of doxorubicin-resistant human myelogenous leukemia cells induced by a composite polymer micelle, Int J Pharm,2011, 420(2): 404-411

Li-Hua Peng, Suk-Ying Tsang, Yasuhiko Tabata, Jian-Qing Gao*, Genetically-manipulated adult stem cells as therapeutic agents and gene delivery vehicle for wound repair and regeneration, J Control Release., 2012.157：321-333

Li-Hua Peng, Kwok-Pui Fung, Ping-Chung Leung, Jian-Qing Gao*, Stem cell based gene delivery system for wound therapy, Drug Discovery Today, 2011,16(21/22):957-966

Ni Li, Li-Hua Peng, Xi Chen, Shinsaku Nakagawa,Jian-Qing Gao*, Transcutaneous vaccines: novel advances in technology and delivery for overcoming the barriers, Vaccine, 2011 Aug 26;29(37):6179-90.

Xing-Lei Yao, Shinsaku Nakagawa*, Jian-Qing Gao*, Current targeting strategies for adenovirus vector in cancer gene therapy,Current Cancer Drug Targets, 2011,7(11):810-825

Xinglei Yao, Yasuo Yoshioka, Tomohiro Morishige, Yusuke Eto, Shogo Narimatsu, Yasuaki Kawai, Hiroyuki Mizuguchi, Jian-Qing Gao, Yohei Mukai, Naoki Okada, Shinsaku Nakagawa, Tumor vascular targeted delivery of polymer-conjugated adenovirus vector for cancer gene therapy, Mol Ther., 2011 Sep;19(9):1619-25.

Cai-Xia He, Ni Li, Yu-Lan Hu, Xiu-Mei Zhu, Hai-Jie Li, Min Han, Pei-Hong Miao, Zhong-Jie Hu, Gang Wang, Wen-Quan Liang, Yasuhiko Tabata, Jian-Qing Gao*, Effective gene delivery to mesenchymal stem cells based on the novel reverse transfection and three-dimensional cell culture system, Pharm Res, 2011, 28(7):1577-1590

Wang WX, Gao JQ, Liang WQ. Chitosan-coated liposomes for intracellular oligonucleotides delivery: Characteristics and cell uptake behavior.Drug Deliv. 2011,Apr;18(3):208-14.

Ying Hu, Yao-Yao Wu, Xiao-Jing Xia, Zheng Wu,Wen-Quan Liang, Jian-Qing Gao^*, Drug-in-adhesive transdermal patch of α-asarone and in vivo pharmacokinetics and efficacy evaluation, Drug Deliv. 2011 Jan;18(1):84-9.

Yu-Lan Hu, Ying-Hua Fu, Yasuhiko Tabata, Jian-Qing Gao*,Mesenchymal stem cells: a promising targeted-delivery vehicle in cancer gene therapy,J Control Release, 2010,147：154-162

Yu-Lan Hu, Jian-Qing Gao*,Potentialneurotoxicity of nanoparticles, Int J Pharm, 2010,394:115-121

Cai-Xia He, Ni Li, Yu-Lan Hu, Xiu-mei Zhu, Hai-Jie Li, Min Han, Pei-Hong Miao, Zhong-Jie Hu, Gang Wang, Wen-Quan Liang, Yasuhiko Tabata, Jian-Qing Gao*, Effective gene delivery to mesenchymal stem cells based on the novel reverse transfection and three-dimensional cell culture system, Pharm Res, in press

Chen J, Yu Z, Chen H, Gao J, Liang W, Transfection efficiency and intracellular fate of polycation liposomes combined with protamine. Biomaterials. 2011 Feb;32(5):1412-8

Cai-Xia He,Zhong-Gui He^*, Jian-Qing Gao^*,Microemulsions as drug delivery system to improve the solubility and the bioavailability of poor water soluble drugs,Expert Opinion on Drug Delivery, 2010 Apr;7(4):445-60.

Jian-Qing Gao*, Qing-Qing Zhao, Teng-Fei Lv, Wu-Ping Shuai, Jun Zhou, Gu-Ping Tang, Wen-Quan Liang, Yasuhiko Tabata, Yu-Lan Hu*, Gene-carried chitosan-linked-PEI induced high gene transfection efficiency, low toxicity and significant tumor-suppressive activity,Int J Pharm, 2010;387(1-2):286-94

Cai-Xia He,Yasuhiko Tabata, Jian-Qing Gao^*, Non-viral gene delivery carrier and its three dimensional transfection system.Int J Pharm, 2010;386:232-242

Yu-Zhe Chen, Xing-Lei Yao, Yasuhiko Tabata, Shinsaku Nakagawa, Jian-Qing Gao*,Gene carriers and transfection systems used in the recombination of dendritic cells for effective cancer immunotherapy, Clinical and Developmental Immunology, Volume 2010, Article ID 565643, doi:10.1155/2010/565643

Ni Li, Yu-Lan Hu, Cai-Xia He, Cheng-Jie Hu, Jun Zhou, Gu-Ping Tang,Jian-Qing Gao*, Preparation, characterization and anti-tumor activity of Ganoderma lucidumpolysaccharide nanoparticle , J Pharm Pharmcol, 2010, 62:139-144

Yuefeng Rao, Feiyue Zheng, Xingguo Zhang, Jianqing Gao and Wenquan Liang, In Vitro Percutaneous Permeation and Skin Accumulation of Finasteride Using Vesicular Ethosomal Carriers, AAPS PharmSciTech, 2008,9(3):860-865

Qing-Qing Zhao,Jin-Liang Chen, Teng-Fei Lv, Cai-Xie He，Gu-Ping Tang,Wen-Quan Liang, Yasuhiko Tabata, and Jian-Qing Gao^*, N/P ratio influence the transfection efficacy and cytotoxicity of Poly(ethylenimine)/Chitosan/DNA complex, Biol Pharm Bull, 2009,432:(4),706-710

Min Han, Cai-Xia He, Qiu-Li Fang, Xiao-Chun Yang, Yuan-Yuan Diao, Dong-Hang Xu, Qiao-Jun He, Yong-Zhou Hu, Wen-Quan Liang, Bo Yang, Jian-Qing Gao*, A novel camptothecin derivative incorporated in nano-carrier induced distinguished improvement in solubility, stability and anti-tumor activity both in vitro andin vivo,Pharm. Res, 2009 Apr;26(4):926-35

Jian-Qing Gao*, Yasuhiro Tsuda, Min Han, Dong-Hang Xu, Naoko Kanagawa, Toshiki Sugita, Yutaka Hatanaka, Yoichi Tani, Hiroyuki Mizuguchi, Yasuo Tsutsumi, Tadanori Mayumi, Naoki Okada,Shinsaku Nakagawa^*, NK cells are migrated and indispensable in the anti-tumor activity induced by CCL27 gene therapy, Cancer Immunol Immunother, 2009 Feb;58(2):291-299.

Jian-Qing Gao*, Naoko Kanagawa, Dong-Hang Xu, Min Han, Toshiki Sugita, Yutaka Hatanaka, Yoichi Tani, Hiroyuki Mizuguchi, Yasuo Tsutsumi, Tadanori Mayumi, Naoki Okada,Shinsaku Nakagawa^*, Combination of two fiber-mutant adenovirus vectors, one encoding the chemokine FKN and another encoding cytokine interleukin 12, elicits notably enhanced anti-tumor responses, Cancer Immunol Immunother, 2008，57（11）：1657-1664

Jian-Qing Gao, Naoko Kanagawa, Yoshiaki Motomura, Tatsuya Yanagawa, Toshiki Sugita, Yutaka Hatanaka, Yoichi Tani, Hiroyuki Mizuguchi, Yasuo Tsutsumi, Tadanori Mayumi, Naoki Okada, and Shinsaku Nakagawa. Cotransduction of CCL27 gene can improve the efficacy and safety of IL-12 gene therapy for cancer. Gene Ther.2007,Mar 14(6):491-502

Jian-Qing Gao,  Yasuhiro Tsuda,  Kazufumi Katayama,  Takashi Nakayama,  Yutaka Hatanaka, Yoichi Tani,  Hiroyuki Mizuguchi,  Takao Hayakawa,  Osamu Yoshie, Yasuo Tsutsumi, Tadanori Mayumi,  Shinsaku Nakagawa. Anti-tumor Effect by　Interleukin-11 Receptor alpha-Locus Chemokine/CCL27, Introduced into Tumor Cells through a Recombinant Adenovirus Vector. Cancer Res. 2003,63(15): 4420-4425

Jian-Qing Gao,Yusuke Eto, Yasuo Yoshioka, Fumiko Sekiguchi,Shinnosuke Kurachi,Tomohiro Morishige, Xinglei Yao, Hikaru Watanabe, Ratima Asavatanabodee,Fuminori Sakurai, Hiroyuki MizuguchiYuka Okada, Yohei Mukai,Yasuo Tsutsumi, Tadanori Mayumi, Naoki Okada, Shinsaku Nakagawa.Effective tumor targeted gene transfer using PEGylated adenovirus vector via systemic administration. J Controlled Release, 2007 Sep 11;122(1):102-10.

Qing-Qing Zhao,Jin-Liang Chen, Teng-Fei Lv, Cai-Xie He，Gu-Ping Tang,Wen-Quan Liang, Yasuhiko Tabata, and Jian-Qing Gao*, N/P ratio influence the transfection efficacy and cytotoxicity of Poly(ethylenimine)/Chitosan/DNA complex, Biol Pharm Bull, 2009,432:(4),706-710

Jian-Qing Gao*,Naoki Okada, Tadanori Mayumi, Shinsaku Nakagawa^*. Immune cell recruitment and cell-based system for cancer therapy.Pharm Res. 2008,25(4):752-768

Xu-Chun Fu, Guo-Ping Wang,Hai-Li Shan, Wen-Quan Liang, Jian-Qing Gao*, Predicting blood-brain barrier penetration from molecular weight and number of polar atoms, Eur J Pharm Biopharm,2008,October, 70(2):462-466

Jin-Liang Chen, Ying Hu, Wu-Ping Shuai, Hai-Liang Chen, Wen-Quan Liang*, Jian-Qing Gao*，Telomerase-targeting antisense oligonucleotides-carried new polycation liposomes enhance the growth inhibition effect on tumor cells，J. Biomed. Mat. Res. Part B. Applied materials,2009 May;89B(2):362-368.

Min Han, Jin-Liang Chen, Ying Hu, Cai-Li HE, Wu-Ping Shuai, Jian-Heng Yu, Hai-Liang Chen, Wen-Quan Liang, Tadanori Mayumi, Nakagawa Shinsaku, and Jian-Qing Gao*, In vitro and in vivo tumor suppressive activity induced by human telomerase transcriptase-targeting antisense oligonucleotides mediated by cationic liposomes, J Biosci Bioeng,2008,106(3):243-247

Qing-Qing Zhao,Jin-Liang Chen,Min Han,Wen-Quan Liang,Yasuhiko Tabata, andJian-Qing Gao*,The Combination of Poly(ethylenimine) and Chitosan Induces High Gene Transfection Efficiency and Low Cytotoxicity,J Biosci Bioeng, 2008,105(1):65-68

Jin-Liang Chen, Hua Wang, Jian-Qing Gao, Hai-Liang Chen, Wen-Quan Liang, Liposomes modified with polycation used for gene delivery: preparation, characterization and transfection in vitro. Int J Pharm. 2007 Oct 1;343(1-2):255-61.

Q.Li, JQ Gao, LY Qiu, FD Cui, Y Jin. Enhanced immune responses induced by vaccine using Sendai virosomes as carrier.Int J Pharm, 2007 Feb 1;329(1-2):117-21

Hai-Liang Chen,Jian-Qing Gao, Fei Wang, Wen-Quan Liang. Preparation, characterization and pharmacokinetics of liposomes encapsulated cyclodextrins inclusion complexes for hydrophobic drugs. Drug Deliv. 2007 May;14(4):201-8.

Jun Liu, Liyan Qiu, Jianqing Gao, Yi Jin. Preparation, characterization and in vivo evaluation of formulationof baicalein with hydroxypropyl-_-cyclodextrin. Int J Pharm, 2006 Apr 7;312(1-2):137-43

Yusuke Eto,Jian-Qing Gao, Fumiko Sekiguchi, Shinnosuke Kurachi, Kazufumi Katayama, Hiroyuki Mizuguchi, Takao Hayakawa, Mitsuko Maeda,Koichi Kawasaki,Yasuo Tsutsumi, Tadanori Mayumi, Shinsaku Nakagawa.PEGylated adenovirus vectors containing RGD peptides on the tip of PEG show high transduction efficiency and protection ability from neutralizing antibodies. J. Gene Med. 2005; 7(5):604-612

Jian-Qing Gao, Toshiki Sugita, Naoko Kanagawa, Keisuke Iida, Naoki Okada,  Hiroyuki Mizuguchi,  Takashi Nakayama, Takao Hayakawa,  Osamu Yoshie,  Yasuo Tsutsumi, Tadanori Mayumi,  Shinsaku Nakagawa.　Anti-tumor Responses Induced by Chemokine CCL19 Transfected Into an Ovarian Carcinoma Model Via Fiber-mutant Adenovirus Vector. Biol.　Pharm.　Bull.　2005; 28(6): 1066-1070

Jian-Qing Gao, Toshiki Sugita, Naoko Kanagawa, Keisuke Iida,Yusuke Eto, Yoshiaki Motomura, Hiroyuki Mizuguchi, Yasuo Tsutsumi, Takao Hayakawa, Tadanori Mayumi, Shinsaku Nakagawa. Single intratumoral injection of interleukin 12 encoded in fiber-mutant adenoviral vector induced the remarkable anti-tumor and anti-metastatic activity in Meth-A fibrosarcoma.Biochem.  Biophys. Res. Commun.　2005,328:1043-1050

Foundations

National Natural Science Foundation of China（30572270），Targeting distribution of cells in vivo through immune factor and delivery system, RMB 200,000, 2006.1-2008.12 (grant principal)

National Natural Science Foundation of China（30873173），Novel gene transfer approach for non-viral carriers based on three dimension construction and its use in gene delivery of stem cells, RBM 300,000，2009.1-2011.12 (grant principal)

National Natural Science Foundation of China（30973648），Development of novel non-viral vectors for dual-transfection of tumor antigen and chemokine receptor into dendritic cells to induce efficient antigen presentation in vivo，RMB 350,000，2010.1-2012.12(grant principal)

Joint Foundation supported by both National Natural Science Foundation of Chinaand JSPS Japan (81011140077) Development of novel non-viral vectors for dual-transfection of tumor antigen and chemokine receptor into dendritic cells to induce efficient antigen presentation in vivo，RMB 110,000，2010.4-2012.12 (grant principal)

Foundation of Science and Technology Department of Zhejiang Province（2006C23015），Development of high efficient extraction approach of anti-tumor products from plants and its nanocarriers for tumor inhibition, RMB 250,000，2006.1-2008.12(grant principal)

Science Fund for Distinguished Young Scholars，Jian-Qing Gao Research Group（R2090176），Natural Science Foundation of Zhejiang Province, RMB 350,000，2010.1-2012.12 (grant principal)

Scientific research fund of Ministry of Health-medical science critical technological program of Zhejiang Province（WKJ2008-2-029），Construction of novel micelles for reversing multidrug resistants in tumor cells, RMB 150,000，2008.7-2011.7 (grant principal)

National Basic Research Program of China(973 program)2009CB930300Studies on the improvement of the related functions for the insoluble drugs through nanotechnology, RMB 39,500,000, 2009.1-2013.12 (grant cadreman)

Joint Center for Development of Novel Transdermal Delivery Systems (Zhejiang University-Nanfang Medical Appliance)，RMB5,000,000，2009.8-2014.8 (grant principal)

Representative Patents:

 Jian-Qing Gao, et al, Construction of PEI-modified chitosan as effective gene carrier, National Patent: ZL200710070441.9, 2009/09/09

Jian-Qing Gao, et al. Transdermal patch of alpha-asarone, National Patent: ZL200610049778.7, 2008/08/27

Jian-Qing Gao, et al. Trandermal  gel of alpha-asarone, National Patent: ZL200610049711.3, 2008/02/27

Wen-Quan Liang, Jian-Qing Gao, A drug-containing gel used for iontophoresis, National Patent: ZL00122182.5, 2004/09/24

Awards

Chinese Embassy Award for the Best Research Paper in Japan,  2003

Science Fund for Distinguished Young Scholars, Zhejiang Province, 2009

New century 151 Distinguished Young Researcher, Zhejiang Province, 2008

Academic Society Activities:

The member of Committee of Pharmaceutics, Chinese Pharmaceutical Association

The member of Chinese Pharmaceutical Association

The member of Japanese Society of Pharmacy

The Editorial Board ofJournal of Cancer Therapy

The Editorial Board of Asian Journal of Pharmaceutical Sciences

The Editorial Board ofChinese Journal of Modern Applied Pharmacy

Councilor ofNational Natural Science Foundation of China for foundation evaluation

Councilor ofthe Ministry of Science and Technology of China for international joint grant evaluation

Councilor of State Food& Drug Administration of China for drug evaluation